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Table 11

Yields of biohydrogen based on experiments' results [31].

Substrate Method Operation Mode Bacteria Microalgae Conditions Biohydrogen Production
Two step Biophotolysis Chlamydomonas reinhardtii D239-40 Light intensity 70 µmol photons/(m2*s) from 2 sides pH 7.5–7.7, 168 h of production 490  ml/L
Crude glycerol Two step Biophotolysis Chlorella sp. pH 6.8, light intensity 48 µmol photons/(m2 * s), sulfur conditions 11.65  ml/L
Direct Biophotolysis Desertifilium sp. IPPAS B-1220 Argon for anaerobic conditions, light intensity 30 µmol photons/(m2*s), shaking at 150 rpm, DCMU addition Maximum production rate after 48 h was 0.348 µmol/(mg Chl h)
Acetate Photo-fermentation Mixed photosynthetic consortium Acidophilic conditions, argon for anaerobic conditions, 34 °C, agitation at 100 rpm, light intensity of 14000 lux presence of MSG and vitamins The optimal conditions were studied and not the actual H2 production value
Acetate Photofermentation Mixed photosynthetic consortium Neutral pH, 30 °C, light intensity 10.25 W/m2, 143.56 ml/L
Acetate Photofermentation, fed batch in pilot scale tubular photobioreactor (80L) Rhodobacter capsulatus Outdoor conditions, mesophilic temperature, pH 8 maintained into a greenhouse, nitrogen, and argon for anaerobic condition, combined artificial and natural light illumination, 30-day process 0.31 mol/(m3 s)
Acetate Batch photofermentation Rhodobacter capsulatus DSM1710 pH 6.4, 30 °C, argon for anaerobic conditions, light intensity 263.6 W/m2 sodium glutamate as nitrogen source 1.04 mmol/(L h)

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